Structure-Activity Relationships of Abscisic Acid Analogs Based on the Induction of Freezing Tolerance in Bromegrass (Bromus inermis Leyss) Cell Cultures

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Structure-Activity Relationships of Abscisic Acid Analogs Based on the Induction of Freezing Tolerance in Bromegrass (Bromus inermis Leyss) Cell Cultures.

The induction of freezing tolerance in bromegrass (Bromus inermis Leyss) cell culture was used to investigate the activity of absisic acid (ABA) analogs. Analogs were either part of an array of 32 derived from systematic alterations to four regions of the ABA molecule or related, pure optical isomers. Alterations were made to the functional group at C-1 (acid replaced with methyl ester, aldehyd...

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Factors Influencing the Induction of Freezing Tolerance by Abscisic Acid in Cell Suspension Cultures of Bromus inermis Leyss and Medicago sativa L.

A 2-gram fresh weight inoculum of bromegrass (Bromus inermis Leyss. culture BG970) cell suspension culture treated with 7.5 x 10(-5) molar abscisic acid (ABA) for 7 days at 25 degrees C survived slow cooling to -60 degrees C. Over 80% of the cells in ABA treated cultures survived immersion in liquid N(2) after slow cooling to -40 or -60 degrees C. In contrast, a 6-gram fresh weight inoculum onl...

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Content during Abscisic Acid Induced Freezing Tolerance in Bromegrass Cells1

Changes in water content and dry weight were determined in control cells and those induced to cold harden in response to abscisic acid (ABA) treatment (7.5 x 10-5 molar). Bromegrass (Bromus inermis Leyss cv Manchar) cells grown in suspension culture at room temperature (230C) for 7 days acclimated to -28°C (LT50) when treated with ABA, or to -50C when untreated. ABA significantly reduced cell g...

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Abscisic Acid increases terrestrial plant cell resistance to hydrostatic pressure.

Cells of the terrestrial plant species bromegrass (Bromus inermis L.) are not naturally adapted to withstand the hydrostatic pressures encountered in aquatic environments. However, after treatment with the natural plant growth hormone abscisic acid (75 micromolar), bromegrass cells survived a hydrostatic pressure of 101.3 megapascals, approximating the limits of ocean depth (10,860 m). The incr...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1992

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.100.4.2024